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Cloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine) Genet. Mol. Biol.
Schlögl,Paulo Sérgio; Santos,André Luis Wendt dos; Vieira,Leila do Nascimento; Floh,Eny Iochevet Segal; Guerra,Miguel Pedro.
Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. Apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil. Thus eight embryogenesis-regulating genes, viz.,ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semiquantitative RT-PCR during embryo development...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seed development; Brazilian Pine; Embryogenesis-regulating genes; Zygotic embryogenesis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100025
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PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze Genet. Mol. Biol.
Schlögl,Paulo Sérgio; Souza,Anete Pereira de; Nodari,Rubens Onofre.
The Araucaria angustifolia (Bert.) O. Kuntze, also named the "paraná pine" (‘pinheiro-do-Paranᒠin Portuguese), is a native conifer species naturally occurring in the Brazilian Tropical Atlantic Forest which in Brazil is mostly limited to the southern Brazilian states of Paraná, Santa Catarina and Rio Grande do Sul. Chloroplast DNA markers (cpDNA) are useful in populational genetic studies because of their low substitution rate and the uniparental transmission. The conservation of cpDNA genes between species has allowed the design of consensus chloroplast primers that have had a great impact on population genetics and phylogenetic studies. In this study we used the polymerase chain reaction technique combined with restriction enzyme fragment length...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haplotypes; Conifer species; Brazilian pine; Genetic diversity.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300020
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